Check out a new press release in phys.org about a recent molecular dynamics (MD) study of the light-harvesting complex of photosystem II (LHCII). The original paper can be found here. The MD study was done by Dr. Yingjie Wang (former Veglia Lab member now Associate Research Investigator at Shenzhen Bay Laboratory).
Analyzing oriented NMR spectra just got a lot easier. Check out our new Sparky plugin recently published in the journal Bioinformatics in collaboration with Woonghee Lee at the University of Wisconsin - Madison. PISA-SPARKY is available with latest version of NMRFAM-SPARKY or as a standalone Python tool than can be downloaded from GitHub here.
Check out our latest work just published in Science Advances. Utilizing calorimetry, NMR and MD simulations this study reveals that a single mutation in protein kinase A disrupts the intramolecular allosteric network to elicit losses in binding cooperativity and changes in substrate specificity.
A collaboration led by researchers at Oak Ridge National Laboratory and the Institut Laue-Langevin in Grenoble, France, has just been published in Science Advances. The study uses neutron diffraction to visualize protons in the structure of Protein Kinase A (PKA-C), providing important insights into hydrogen-bonding networks and protonation states that underpin allostery and catalysis.
In a world’s first, scientists in the Veglia lab have imaged the first PISA wheel in the Sarcolipin cluster. The image was a result of a painstaking 20 minutes of acquisition time on a 600 MHz NMR spectrometer and a whopping 514 KB of data. To get an idea of that scale, that’s the time it takes to drink a coffee and just over a third of a floppy disk. The results were very surprising. One confused scientist in the group thought he was seeing some sort of space bagel.
More to come…
Losing sleep over the role of conformational entropy and allosteric cooperativity in the enzymatic cycles of kinases? If so, our new paper utilizing calorimetry, NMR and MD simulations of the conformational landscape of cAMP-dependent protein kinase A (PKA-C) might be worth a read.
Congratulations to Daniel for being awarded an American Heart Association Postdoctoral Fellowship. His project will focus on gaining a structural and functional understanding of how mutations and post-translational modifications of phospholamban lead to dilated cardiomyopathy.
On April 9, 2018 an article in Current Protocols in Chemical Biology came out titled "ALARM NMR for HTS Triage and Chemical Probe Validation", authored by Jayme L. Dahlin et.al. in collaboration with the Veglia Lab. See the article here.
On Friday, January 19, 2018, Dr. Sarah E.D. Nelson successfully defended her thesis. Sarah has been working in the Veglia and Thomas laboratories on various projects involving calcium regulation by phospholamban and sarcolipin, as well as calmodulin/ryanodine receptor binding and regulation. She studied both solution and solid state NMR during her time at UMN.
We wish her all the best as she begins work as a Postdoctoral Associate!
Be sure to check out the Journal of Magnetic Resonance, volume 285, December 2017. The publication from Gopinath T. et al is titled "1H-detected MAS solid-state NMR experiments enable the simultaneous mapping of rigid and dynamic domains of membrane proteins". The feature figure demonstrates a new pulse sequence for simultaneous acquisition of riHSQC and cpHSQC. You can link to the full publication here.
This week, a news article came out by Twin Cities Business which highlights the work done by Profs. Michael Bowser, Gianluigi Veglia, and Joseph Metzger. They have secured a patent for an interesting new scientific method which makes use of DNA "adaptamers" and their ability to target specific mechanisms related to heart failure.
To see the full story, check out: http://tcbmag.com/news/articles/2017/september/u-of-m-lands-patent-for-u...
The Minnesota NMR Center is now accepting registrations for the 2017 Advanced Hands-On NMR Workshop: From Protein Structure Determination to Drug Design.
Dr. Geoffrey Li started in Dr. Veglia's laboratory at University of Minnesota in 2012. He completed his Masters degree in Chemistry in 2013, followed by his PhD in Chemistry this month. Geoffrey has made many contributions to the projects he had worked on in his 5 years with the group. He has taken a position at Vanderbilt University, in the lab of Chuck Sanders, and we wish him the best!
The 61st Annual Biophysical Society Meeting will be held in New Orleans, LA from February 11-15, 2017. The abstract deadline is October 3, 2016 and Early Registration deadline is January 9, 2017. If you will attend, you must register during early registration to capture the reduced rate.
Jonggul Kim and Cristina Olivieri are representing the research group at the "Kinases and Pseudokinases: Spines, Scaffolds and Molecular Switches" symposium at University of California, San Diego December 2015. The conference is organized by the American Society for Biochemistry and Molecular Biology.
Jonggul gave a talk "Signaling in the catalytic subunit of protein kinase A via hydrophobic motifs", while Cristina presented a poster "Conformational landscape of protein kinase inhibitor with and without protein kinase A ".
American Heart Association offers funding opportunities for graduate students and postdocs interested in initiating careers in cardiovascular and stroke research.